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秦丽娜研究员

时间:2019-03-04浏览:2311


Lina Qin

Principal Investigator

Professor of Microbiology, Biochemistry and Molecular Biology

College of Life Sciences, Fujian Normal University (Qishan Campus),

Fuzhou City, Fujian Province 350117, China



Lina Qin is a professor in College of Life Sciences at Fujian Normal University. She received her Bachelor's degree in Biotechnology from Shanxi University (2005) and received her Master’s degree in Biochemistry and Molecular Biology from Fujian Normal University (2008).She earned her PhD degree in biochemistry and molecular biology from Institute of Microbiology, Chinese Academy of Sciences (2012). After a short postdoctoral training in the Institute of Process Engineering, Chinese Academy of Sciences (2012-2013), she joined in Energy Biosciences Institute at UC Berkeley in the USA as a postdoctoral scholar (2014-2017). She then went back to China and started her independent career in Fujian Normal University in August 2017. She has become a full professor since January 2019.


Research Areas


Topic 1:Molecular mechanism of protein secretion in saprophytic filamentous fungi


     Protein biosynthesis, modification, processing and secretion are one of the critical basic scientific questions in life sciences. As the main decomposer of lignocellulosic biomass, saprophytic filamentous fungus has evolved a large capacity of protein secretion. For example, some industrial mutants of Trichoderma reesei have a protein secretion capacity of over 100 g/L. However, little is known about the molecular mechanism behind this property. Our research group will focus on the secretion pathway of the plant cell wall degrading associated proteins in the model filamentous fungus Neurospora crassa and Trichoderma reesei by using variety of molecular, genetic and biochemical techniques developed for these two organisms. Understanding the molecular basis of the protein secretion in model systems will provide new clues and strategies for industrial strain engineering to improve the protein expression and secretion.


Topic 2:Characterization of novel enzymes from thermophilic fungi and development of thermophilic fungi as new sources for production of industrial enzymes


    Biorefinery is the sustainable processing biomass into a variety of bio-products. Many of these processes require enzymes which are stable at high temperature to enhance mass transfer, reduce substrate viscosity and lower risk of contamination. Many thermophilic fungi can secrete thermostable biomass-degrading enzymes including lignocellulases, proteases, amylases, laccases, chitinases, lipases and esterases, which holds a great promise in industrial application. In addition, as a natural plant-biomass degrader, thermophilic fungi have evolved an efficient protein secretion system. Therefore, they are also potentially likely to be developed into cell factories to produce heterologous recombinant proteins at elevated temperatures. Some unpublished data from Louis Glass’s lab (UC Berkeley)indicated that Chaetomium thermophilum displayed substantially higher cellulose degrading activity and Melanocarpus albomyces exhibited a considerable total extracellular protein production. Both C. thermophilum and M. albomyces are closely related to N. crassa. Our research group will currently focus on characterization of novel enzymes from C. thermophilum and developing M. albomyces as an excellent host for protein expression by using variety of knowledge and technology developed in N. crassa and T. reesei.


Topic 3:Antifungal mechanism of new discovered antifungal compounds


    The development of bioinformatics, omics, mass spectrometry and high-throughput screening techniques inspired more and more novel nature products isolated and identified. Many of these natural compounds have the antifungal activities with unknown mode of action. Our group will collaborate with the research group, whose research interest is discovery of new antifungal natural products, to further study the antifungal mechanism of these novel compounds in the model filamentous fungi N. crassa by using variety of molecular, genetic and biochemical techniques developed for N. crassa. We currently focus on a broad spectrum antifungal compound HSAF, which is isolated from Lysobacter bacteria by Liangcheng Du’s group from the university of Nebraska-Lincoln.


Publications


  1.  Fei Gao, Zhenzhen Hao, Xianhua Sun, Lina Qin, Tong Zhao, Weiquan Liu, Huiying Luo, Bin Yao and Xiaoyun Su*. A versatile system for fast screening and isolation of Trichoderma reesei cellulase hyperproducers based on DsRed and fluorescence-assisted cell sorting. Biotechnology for Biofuels, 2018; 11: 261. doi: 10.1186/s13068-018-1264-z

  2. Lina Qin*, Xiangzhang Jiang, Zhiyang Dong, Jianzhong Huang and Xiuzhen Chen*. Identification of two integration sites in favor of transgene expression in Trichoderma reesei, Biotechnology for Biofuels, 2018,11142. DOI : 10.1186/s13068-018-1139-3.

  3.  Xianhua Sun, Xianli Xue, Mengzhu Li, Fei Gao, Zhenzhen Hao, Huoqing Huang, Huiying Luo, Lina Qin*, Bin Yao*, Xiaoyun Su*. Efficient co-production of mannanase and cellulase by transformation of a codon-optimized endo-mannanase gene from Aspergillus niger into Trichoderma reesei. Journal of Agricultural and Food Chemistry. 2017 Dec 20;65(50):11046-11053. doi: 10.1021/acs.jafc.7b05114.

  4. Lina Qin, Vincent W. Wu, N. Louis Glass*. Deciphering the Regulatory Network between the SREBP pathway and Protein Secretion in Neurospora crassa. mBio, 2017, 8(2): e00233-17. Doi: 10.1128/mBio.00233-17.

  5.  Yi Xiong, Vincent W. Wu, Andrea Lubbe, Lina Qin , Siwen Deng , Megan Kennedy, Diane Bauer, Vasanth R. Singan, Kerrie Barry, Trent R. Northen, Igor V. Grigoriev, N. Louise Glass*. A Fungal Transcription Factor Essential for Starch Degradation Regulates Primary Carbon and Nitrogen Metabolism. Plos Genetics, 2017, 13(5): e1006737. doi:10.1371/journal.pgen.1006737

  6.  Lori B. Huberman, Jason Liu, Lina Qin and N. Louise Glass*. Regulation of the lignocellulolytic response in filamentous fungi. Fungal Biology Reviews, 2016, 30:101-111.

  7.  Morgann C Reilly, Lina Qin, James P Craig, Trevol L Starr and N Louise Glass*.Deletion of homologs of the SREBP pathway results in hyper-production of cellulasesin Neurospora crassa and Trichoderma reesei. Biotechnology for Biofuels 2015, 8:121. doi: 10.1186/s13068-015-0297-9.

  8.  Fei Chen, Xiuzhen Chen, Xiaoyun Su, Lina Qin, Zhenbang Huang, Yong Tao and Zhiyang Dong*. An Ime2-like mitogen-activated protein kinase is involved in cellulaseexpression in the filamentous fungus Trichoderma reesei, Biotechnology letters, 2015,37(10): 2055-62.

  9.  Zhenbang Huang, Xiuzhen Chen, Lina Qin, Hongqing Wu, Xiaoyun Su andZhiyang Dong*. A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei, Biochemicaland biophysical research communications, 2015, 460(3): 663-669.

  10.  Fei Chen, Xiuzhen Chen, Lina Qin, Yong Tao and Zhiyang Dong*. Characterizationand homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reeseiBiochemical and biophysical research communications, 2015,459(2): 184-188.

  11.  Xiao-yun Su*, Lina Qin*, Zhi-yang Dong. RNAi-mediated gene silencing in Trichoderma--principles and applications. A chapter in the book of ‘Trichoderma:Their uses and applications in bioengineering and biotechnology’, 2014, Doi:10.1016/B978-0-444- 59576-8.00015-1 Elsevier .

  12. Lina Qin, Fu-rong Cai, Xin-rui Dong, Zhen-bang Huang, Yong Tao, Jian-zhongHuang*, Zhi-yang Dong*. Improved production of heterologous lipase in Trichoderma reesei by RNAi mediated gene silencing of an endogenic highly expressed gene. Bioresource Technology, 2012, 109:116-122.




姓 名

秦丽娜

所属系部


职 称

研究员

最高学位

博士

职 务


联系电话

17750318602

电子邮箱

qinln@fjnu.edu.cn

办公室或实验室地 址

12号理工楼101房间


个人简介或履历

1983年生,山西晋城人。2005年毕业于山西大学,获生物技术专业学士学位;2008年毕业于福建师范大学,获生物化学与分子生物学专业硕士学位;2012年毕业于中国科学院微生物研究所,获生物化学与分子生物学专业博士学位。2012年至2013年在中国科学院过程工程研究所进行博士后研究;2014年至2017年在美国加州大学伯克利分校进行博士后研究。20178月正式加入福建师范大学亚博yabo888vip网页登录。

主要从事丝状真菌分子遗传学研究,在微生物领域权威期刊mBio, Plos Genetics, Biotechnology for Biofuels等刊物上发表SCI论文14篇;授权两项国家发明专利;主持2项国家自然科学基金项目;主持福建省高校杰出青年科研人才计划项目1项;曾获加州大学伯克利分校Philomathia博士后基金(UC Berkeley Philomathia Postdoctoral Fellowship)资助。


研究方向

研究方向一: 腐生丝状真菌蛋白分泌分子机制研究

蛋白质的生物合成、修饰加工以及分泌途径是生命科学领域重要的基础科学问题。腐生丝状真菌作为自然界木质纤维素类物质的主要分解者,在长期的进化过程中,形成了强大的蛋白分泌能力。如:里氏木霉(Trichoderma reesei)和嗜热毁丝菌(Myceliophthora thermophila)的一些工业突变株,其蛋白分泌能力可达100 g/L,然而这种强大蛋白分泌能力背后的分子机制却缺乏系统研究。本课题组将以模式丝状真菌粗糙脉孢霉(Neurospora crassa)和里氏木霉为研究对象,采用分子遗传学手段、活细胞成像技术、分泌蛋白组学分析以及特定蛋白的脉冲跟踪技术等研究方法全面系统地揭示腐生丝状真菌的蛋白分泌机制。我们的研究将有助于扩充丝状真菌蛋白分泌的知识架构,同时也为丝状真菌在生物质降解利用、推进自然界碳素循环等方面的研究奠定一定的理论基础。


研究方向二:嗜热真菌酶基因资源挖掘及嗜热真菌高效蛋白分泌表达系统的开发和应用

嗜热丝状真菌有着优良的酶基因资源和发酵特性,却极少被研究和开发。如许多生物质降解类嗜热真菌可分泌多种热稳定性极好的的生物质降解酶,包括纤维素酶、蛋白酶、淀粉酶、漆酶、几丁质酶、脂肪酶和酯酶等。将这些酶开发为酶制剂产品在养殖、种植、环境保护、食品及保健品等领域有着非常广泛的应用价值。另外,嗜热真菌的发酵温度通常高于50℃,在发酵过程中可以有效降低发酵反应器被其他微生物物种污染的风险。因此,本课题组将对一些已完成基因组草图,但至今仍未开发的嗜热丝状真菌,如Chaetomium thermophilum, Corynascus thermophilus, Humicola brevis, Melanocarpus albomyces, and Stilbella thermophila 等进行产酶性状评估,对其中有工业应用价值的酶基因进行克隆并在工业应用菌株里氏木霉中进行异源表达;同时利用本课题组前期在模式丝状真菌中的众多技术方法积累,对一些具有蛋白分泌潜力的嗜热真菌进行遗传改造,构建高效的嗜热真菌蛋白分泌表达系统。


研究方向三:新型抗真菌小分子化合物的作用机制研究

随着生物信息学、组学、质谱学以及活性物质的高通量筛选技术的发展,越来越多新型的、具有抗真菌功能的活性物质被分离和鉴定出来。本课题组在模式丝状真菌分子遗传学方面有着很好的积累,将与国内外致力于天然产物挖掘的实验室开展合作,对其筛选分离到的抗真菌活性物质背后的抗真菌机理进行系统研究。


科研项目

  1. 国家自然科学基金(青年项目):翻转酶(Flippase)在丝状真菌蛋白分泌过程中的作用机制研究,318000602019.1-2021.12,在研,主持。

  2. 国家自然科学基金(应急项目):转录因子xyr1clr-2在丝状真菌降解植物细胞壁过程中的功能演变研究,317410022018.1-2018.12,在研,主持。

  3. 福建省高校杰出青年科研人才培育计划项目,2019.1-2020.12




论文发表

  1. Fei Gao, Zhenzhen Hao, Xianhua Sun, Lina Qin, Tong Zhao, Weiquan Liu, Huiying Luo, Bin Yao and Xiaoyun Su*. A versatile system for fast screening and isolation of Trichoderma reesei cellulase hyperproducers based on DsRed and fluorescence-assisted cell sorting. Biotechnology for Biofuels, 2018; 11: 261. doi: 10.1186/s13068-018-1264-z

  2. Lina Qin*, Xiangzhang Jiang, Zhiyang Dong, Jianzhong Huang and Xiuzhen Chen*. Identification of two integration sites in favor of transgene expression in Trichoderma reesei, Biotechnology for Biofuels, 2018,11142. DOI : 10.1186/s13068-018-1139-3.

  3. Xianhua Sun, Xianli Xue, Mengzhu Li, Fei Gao, Zhenzhen Hao, Huoqing Huang, Huiying Luo, Lina Qin*, Bin Yao*, Xiaoyun Su*. Efficient co-production of mannanase and cellulase by transformation of a codon-optimized endo-mannanase gene from Aspergillus niger into Trichoderma reesei. Journal of Agricultural and Food Chemistry. 2017 Dec 20;65(50):11046-11053. doi: 10.1021/acs.jafc.7b05114.

  4. Lina Qin, Vincent W. Wu, N. Louis Glass*. Deciphering the Regulatory Network between the SREBP pathway and Protein Secretion in Neurospora crassa. mBio, 2017, 8(2): e00233-17. Doi: 10.1128/mBio.00233-17.

  5. Yi Xiong, Vincent W. Wu, Andrea Lubbe, Lina Qin , Siwen Deng , Megan Kennedy, Diane Bauer, Vasanth R. Singan, Kerrie Barry, Trent R. Northen, Igor V. Grigoriev, N. Louise Glass*. A Fungal Transcription Factor Essential for Starch Degradation Regulates Primary Carbon and Nitrogen Metabolism. Plos Genetics, 2017, 13(5): e1006737. doi:10.1371/journal.pgen.1006737

  6. Lori B. Huberman, Jason Liu, Lina Qin and N. Louise Glass*. Regulation of the lignocellulolytic response in filamentous fungi. Fungal Biology Reviews, 2016, 30:101-111.

  7. Morgann C Reilly, Lina Qin, James P Craig, Trevol L Starr and N Louise Glass*.Deletion of homologs of the SREBP pathway results in hyper-production of cellulasesin Neurospora crassa and Trichoderma reesei. Biotechnology for Biofuels 2015, 8:121. doi: 10.1186/s13068-015-0297-9.

  8. Fei Chen, Xiuzhen Chen, Xiaoyun Su, Lina Qin, Zhenbang Huang, Yong Tao and Zhiyang Dong*. An Ime2-like mitogen-activated protein kinase is involved in cellulaseexpression in the filamentous fungus Trichoderma reesei, Biotechnology letters, 2015,37(10): 2055-62.

  9. Zhenbang Huang, Xiuzhen Chen, Lina Qin, Hongqing Wu, Xiaoyun Su andZhiyang Dong*. A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei, Biochemicaland biophysical research communications, 2015, 460(3): 663-669.

  10. Fei Chen, Xiuzhen Chen, Lina Qin, Yong Tao and Zhiyang Dong*. Characterizationand homologous overexpression of an N-acetylglucosaminidase Nag1 from Trichoderma reeseiBiochemical and biophysical research communications, 2015,459(2): 184-188.

  11. Xiao-yun Su*, Lina Qin*, Zhi-yang Dong. RNAi-mediated gene silencing in Trichoderma--principles and applications. A chapter in the book of ‘Trichoderma:Their uses and applications in bioengineering and biotechnology’, 2014, Doi:10.1016/B978-0-444- 59576-8.00015-1 Elsevier .

  12. Lina Qin, Fu-rong Cai, Xin-rui Dong, Zhen-bang Huang, Yong Tao, Jian-zhongHuang*, Zhi-yang Dong*. Improved production of heterologous lipase in Trichoderma reesei by RNAi mediated gene silencing of an endogenic highly expressed gene. Bioresource Technology, 2012, 109:116-122.


著作出版



专利发明

  1. 董志杨,秦丽娜;高通量筛选高效表达外源蛋白的重组里氏木霉的方法(授权),专利号:ZL201210271826.2

  2. 董志杨,秦丽娜;来源于里氏木霉的一个蛋白质及其基因的用途(授权),专利号:ZL201210264070.9


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